Basma K Mahmoud1,
Ashraf NE Hamed1 
,
Mamdouh N Samy1,
Ehab M Mostafa2,3,
Amira Samir Wanas1,4,
Mohamed M Radwan4,
Mahmoud A Elsohly4,5,
Mohamed Salah Kamel1,6
For correspondence:- Ashraf Hamed Email: ashrafnag@mu.edu.eg Tel:+208623477-59
Accepted: 18 November 2019 Published: 31 December 2019
Citation: Mahmoud BK, Hamed AN, Samy MN, Mostafa EM, Wanas AS, Radwan MM, et al. Phytochemical composition and antimicrobial properties of Markhamia platycalyx (Baker) Sprague leaf. Trop J Pharm Res 2019; 18(12):2623-2631 doi: 10.4314/tjpr.v18i12.23
© 2019 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To isolate new antimicrobial agents from the leaves of Markhamia platycalyx (Baker) Sprague and assess their phytochemical characteristics and antimicrobial activity.
Methods: Different chromatographic and spectroscopic techniques (NMR and ESI-MS) were applied for the identification of antimicrobial compounds. Agar-well diffusion technique was used for determination of antimicrobial activity. Anti-HCV effects were investigated using VITROS Anti-HCV assay.
Results: Eighteen compounds were isolated for the first time from this genus. These were phytol, n-octacosanoic acid (OCTA), tormentic acid and β-sitosterol-3-O-(6'-O-heptadecanoyl)-β-D-glucopyranoside. The other compounds were β-sitosterol, ursolic acid (URSA), oleanolic acids, pomolic acid (POMA), 2-epi-tormentic and β-sitosterol-3-O-β-D-glucopyranoside. However, stigmasterol and acteoside, which were seen in previous studies, were also present. Total ethanol extract (TEE) was the most effective against Escherichia coli, with the lowest minimum inhibitory concentration (MIC) of 1.0 µg/mL. Acteoside, URSA and 2-epi-tormentic acid showed the highest antibacterial effect on Pseudomonas aeruginosa while 2-epi-tormentic acid and acteoside produced the least MIC on Candida glabrata. These effects were superior to those produced by standard antibiotics. However, 2-epi-tormentic acid and β-sitosterol-3-O-β-D-glucopyranoside had no anti-HCV effects.
Conclusion: Due to the good antimicrobial properties of Markhamia platycalyx, it is a potential source of new antimicrobial drugs.
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